Método de determinación de nucleótidos en alimentos mediante cromatografía líquida de pares iónicos con detección por fotodiodos acoplada a espectrometría de masas
Método de determinación de nucleótidos en alimentos mediante cromatografía líquida de pares iónicos con detección por fotodiodos acoplada a espectrometría de masas de tiempo de vuelo con ionización dual operando en modo negativo (HPLC/ESI-APCI-TOF-MS). El método permite la determinación rápida de los cinco nucleótidos monofosfato autorizados en alimentos infantiles (citidina 5'-monofosfato, uridina 5'- monofosfato, adenosina 5'-monofosfato, inosina 5'-monofosfato y guanosina 5'-monofosfato), y comprende la precipitación previa de las proteínas de los alimentos y el análisis directo de los nucleótidos en el extracto resultante por HPLC de pares iónicos usando elución isocrática. La recuperación obtenida para muestras fortificadas fue satisfactoria para todos los analitos. El procedimiento propuesto permite la determinación de los únicos nucleótidos autorizados y se ha aplicado con éxito al análisis de diferentes muestras de alimentos infantiles y funcionales, tales como purés, cereales y potitos.
![Nucleótidos](data:image/png;base64,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)