Dr. PILAR COY | Full Professor
Dr. Pilar Coy Fuster is a Full Professor at the Physiology Deparment of the Veterinary Faculty of the University of Murcia.
| Contact | pcoy@um.es | +(34) 868 884789 |
|---|---|
| University | University of Murcia |
| Department | Physiology |
| Faculty | Veterinary |
| Research fields | Reproductive fluids, in vitro fertilization, oviduct, polyspermy, epigenetics |
Biography
Education
| Degree in Veterinary | University of Murcia | 1987 |
|---|---|---|
| PhD, Doctor in Veterinary Medicine | University of Murcia | 1991 |
| Diplomate in Animal Reproduction | European Collegue of Animal Reproduction | 2001 |
Currivulum Vitae Summary
After finishing her PhD at the University of Murcia, the first ten years of her postdoctoral scientific career focused on the construction of a solid research group at the Physiology Department of the Veterinary Faculty. In order to do so, she learned different protocols and techniques through research stays in UC Davis (California, USA, 1992), Cambridge (UK, 1994) and Knoxville (Tennessee, USA, 2002). Meanwhile, she obtained the funding and equipment needed for in vitro embryo production. She started working in porcine species but later she included cattle and other species of veterinary interest.
Between 2005 and 2015, her research focused on deciphering the different physiological mechanisms involved in sperm-oocyte interaction and fertilization, aiming to increase the in vitro fertilization rates in domestic mammals. She discovered an oviductal mechanism mediated by the specific oviductal glycoprotein OVGP1, which contributed to polyspermy regulation and increased fertilization rates in the sow and the cow. This discovery lead to a patent and a publication in PNAS (2008), among other achievement. She and her team demonstrated that this mechanism is evolutionarily conserved in ungulates (goat, sheep, cow and pig) but it is absent in human and some rodents.
Additionally, she described the role of the plasminogen/plasmin system in the oviduct, and performed genomic and proteomic studies of the oviductal environment, trying to identify the proteins involved in the success of the fertilization. Currently, her research focuses on the use of reproductive fluids to improve the assisted reproductive technologies and the health of the offspring, both at phenotypical and epigenetic level. To this end, she and her team have created the first biobank of animal fluids, which has led to the creation of a website for the distribution of the different fluids, a patent extended to Europe, USA and Brazil, and the registration of two trademarks at euro level (Embryocloud and NaturARTs), as well as the first collection of human reproductive fluids stored at a registered biobank.
From the teaching point of view, she designed and launched in 2004 the specific Master and PhD programmes in Biology and Technology of Reproduction in the University of Murcia, which have been awarded with Mentions of Quality and Excellence by the Spanish Ministry of Education. In the framework of these programmes, researchers and scientists all over the world have visited the University of Murcia and participated in the courses, and established cooperations with Dr. Coy’s group. In 2015, she applied for a Joint European PhD Programme, in the framework of the ITN Marie Sklodowska-Curie excellence network (Horizon 2020) that was funded with 3.8 million euros and successfully finished in 2019 with 15 new PhD fellows in the field of reproductive biology and technology.
Currently, she is associate editor of the scientific journals Molecular Reproduction and Development and Molecular Human Reproduction.
Quality of Teaching, Research and Transfer Periods
Dr. Coy has been recognized by the Spanish Ministry for the quality in her six 'five-year teaching-periods', five 'six-year research-periods', and one 'six-year transfer-period'. She has supervised 13 PhDs, 7 of them in the last 10 years, 4 bachelor's thesis, 7 DEAs and 29 master’s thesis.
She is the author of 130 publications included in the Web of Science, 85 of them being full articles, and most of them in Journals in the Q1 of their JCR area. Her h-index is 31 in the Web of Science and 38 in Google Scholar, where her total citation number is 4070 and the average citation per year, in the last five years was 358.
Publications
Publications included in the Science Citation Index (SCI) of the Institute for Scientific Information (ISI)
LOPES JS, IVANOVA E, RUIZ S, ANDREWS S, KELSEY G, COY P. Effect of superovulation treatment on oocyte´s DNA methylation. International Journal of Molecular Sciences. 2022, 23, 16158. doi.org/10.3390/ijms232416158 IF: 6,208.
ROMERO-AGUIRREGOMEZCORTA J, RAMÍREZ LL, ORTÍN A, RAMIS G, L ROMAR R, COY P. Differences in weight, hierarchy, and incidence of lameness between two groups of adult pigs derived from assisted reproductive technologies. Animals. 2022, 12, 3578. doi.org/10.3390/ani12243578
LI Y, SENA LOPES J, COY P, RIVERA RM. Spontaneous and ART-induced large offspring syndrome: similarities and differences in DNA methylome. Epigenetics. 2022. doi.org/10.1080/15592294.2022.2067938 IF: 4,584.
COY P, ROMAR R, ROMERO-AGUIRREGOMEZCORTA J. The embryo culture media in the era of epigenetics: is it time to go back to nature? Animal Reproduction. 2022;19(1):e20210132. doi.org/10.1590/1984-3143-AR2021-0132
PARÍS-OLLER, C. MATÁS, R. ROMAR, J. S. LOPES, J. GADEA, S. CÁNOVAS, P. COY. Growth analysis and blood profile in piglets born by Embryo transfer. Research in Veterinary Science. January 2022. Volume 142, Pages 43-53. doi.org/10.1016/j.rvsc.2021.11.010
PARÍS-OLLER, C. SORIANO-ÚBEDA, R. BELDA-PÉREZ, L. SARRÍAS-GIL, J.S. LOPES, A. CANHA-GOUVEIA, L.A. VIEIRA, J. GADEA, F.A. GARCÍA-VÁZQUEZ, R. ROMAR, S. CÁNOVAS, P. COY. Reproductive fluids, added to the culture media, contribute to minimizing phenotypical differences between in vitro-derived and artificial insemination-derived piglets. Journal of Developmental Origins of Health and Disease. 2021. doi.org/10.1017/S2040174421000702
LOPES JS, SORIANO-ÚBEDA C, PARÍS-OLLER C, NAVARRO SERNA S, CERON JJ, COY P. Year-long phenotypical study of calves derived from different assisted-reproduction technologies. Frontiers in Veterinary Science, section Animal Reproduction – Theriogenology. 2021. doi.org/10.3389/fvets.2021.739041
CANHA-GOUVEIA A, PRIETO-SÁNCHEZ MT, SÁNCHEZ-FERRER ML, MOLLÁ M, PARÍS-OLLER E, SORIANO C, LANDERAS J, COY P. Physicochemical and Functional Characterization of Woman Reproductive Fluids: First Two Babies Born After Addition of Fluids of Their Moms to Embryo Culture Media. Frontiers in Physiology (Developmental Physiology). August 2021 | Volume 12 | Article 710887. doi.org/10.3389/fphys.2021.710887
CANOVAS S, IVANOVA E, HAMDI M, PEREZ-SANZ F, RIZOS D, KELSEY G AND COY P. Culture medium and sex drive epigenetic reprogramming in preimplantation bovine embryos. International Journal of Molecular Sciences. 2021 Jun 15;22(12):6426. doi.org/10.3390/ijms22126426
PARIS-OLLER E, NAVARRO-SERNA S, SORIANO-ÚBEDA C; SENA LOPES J, MATAS, C, RUIZ S, LATORRE R, LÓPEZ-ALBORS O, ROMAR R, CÁNOVAS S, COY P. Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM. Journal of Animal Science and Biotechnology. 2021. JASB-D-20-00543. doi.org/10.21203/rs.3.rs-42227/v1
GARCÍA-MARTÍNEZ S, GADEA J, COY P, ROMAR R. Addition of exogenous proteins detected in oviductal secretions to in vitro culture medium does not improve the efficiency of in vitro fertilization in pigs. Theriogenology. 2020:157: 490-497. doi.org/j.theriogenology.2020.08.017
SENA LOPES J, ALCÁZAR-TRIVIÑO E, SORIANO-ÚBEDA C, HAMDI M, CÁNOVAS S, RIZOS D, COY P. Reproductive outcomes and endocrine profile in artificially inseminated versus embryo transferred cows. Animals. 2020, 10, 1359. doi.org/10.3390/ani10081359
GARCÍA-MARTÍNEZ S, LATORRE R, SÁNCHEZ-HURTADO MA, SÁNCHEZ-MARGALLO FM, BERNABÒ N, ROMAR R, LÓPEZ-ALBORS O, COY P. Mimicking the temperature gradient between the sow’s oviduct and uterus improves in vitro embryo culture output. Molecular Human Reproduction (2020) doi.org/10.1093/molehr/gaaa053
IVANOVA E, CANOVAS S, GARCIA-MARTINEZ S, ROMAR R, LOPES J; RIZOS D; SANCHEZ-CALABUIG MJ, KRUEGER F, ANDREWS S; PEREZ-SANZ F; KELSEY G, COY P. DNA methylation changes during preimplantation development reveal inter-species differences and reprogramming events at imprinted genes. Clinical Epigenetics (2020) 12:64. doi.org/10.1186/s13148-020-00857-x6
ALCÂNTARA-NETO AS, FERNANDEZ-RUFETE M, CORBIN E, TSIKIS G, UZBEKOV R, GARANINA AS, COY P, ALMIÑANA C, MERMILLOD P. Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilization. Reproduction, Fertility and Development. (2020) Feb;32(4):409-418. doi.org/10.1071/rd190583
CANHA-GOUVEIA A, PARADELA A, RAMOS-FERNÁNDEZ A, PRIETO-SÁNCHEZ MT, SÁNCHEZ-FERRER ML, CORRALES F, COY P. Which low-abundance proteins are present in the human milieu of gamete/embryo maternal interaction? International Journal of Molecular Sciences. (2019) 20, 5305. doi.org/10.3390/ijms20215305
SENA LOPES J, CANHA-GOUVEIA A, PARÍS-OLLER E, COY P. Supplementation of bovine follicular fluid during in vitro maturation increases oocyte cumulus expansion, blastocyst developmental kinetics, and blastocyst cell number. Theriogenology. (2019) 126:222-229. doi.org/theriogenology.2018.12.010
ROMAR R, CANOVAS S, MATAS C, GADEA J, COY P. Pig in vitro fertilization: where are we and where do we go? Theriogenology. (2019) 137:113-121. doi.org/theriogenology.2019.05.045
GARCÍA-VÁZQUEZ FA, SORIANO-ÚBEDA C, LAGUNA-BARRAZA R, IZQUIERDO-RICO MJ, NAVARRETE FA, VISCONTI PE, GUTIÉRREZ-ADÁN A, COY P. Tissue plasminogen activator (tPA) of paternal origin is necessary for the success of in vitro but not of in vivo fertilisation in the mouse. Reproduction, Fertility and Development. (2018) doi.org/10.1071/RD18175
MARTINEZ-SOTO JC, LANDERAS J, MOLLA M, MONDEJAR I, NICOLAS M, FERNANDEZ-OLMEDILLA L TRABALON M, COY P, GADEA J. Total urokinase-type plasminogen activator (uPA) levels in seminal plasma are associated with positive Assisted Reproductive Technology outcomes. Journal of Assisted Reproduction and Genetics. (2018) 35: 1091-1101. doi.org/s10815-018-1164-y
GARCÍA-MARTÍNEZ S, SÁNCHEZ-HURTADO MA, GUTIÉRREZ H, SÁNCHEZ-MARGALLO FM, ROMAR R, LATORRE R, COY P, LÓPEZ-ALBORS O. Mimicking physiological O2 tension in the female reproductive tract improves Assisted Reproduction outcomes in pig. Molecular Human Reproduction. (2018) 24: 260-270. doi.org/10.1093/molehr/gay008
PEREZ-CEREZALES S, RAMOS-IBEAS P, AVILES M, COY P, RIZOS D, GUTIERREZ-ADAN A. The oviduct: from sperm selection to the epigenetic landscape of the embryo. Biology of Reproduction. (2018) 98: 262-276. doi.org/biolre/iox173
FONSECA BALVÍS N, GARCIA-MARTINEZ S, PÉREZ-CEREZALES S, IVANOVA E, GOMEZ-REDONDO I, HAMDI M, RIZOS D, COY P, KELSEY G, GUTIERREZ-ADAN A. Cultured bovine embryo biopsy conserves methylation marks from original embryo. Biology of Reproduction. (2017) 97(2):189-196. doi.org/10.1093/biolre/iox077
CÁNOVAS S, ROSS PJ, KELSEY G, COY P. DNA methylation dynamics in preimplantation embryos: the epigenetic impact of Assisted Reproductive Technologies . BioEssays. (2017) 1700106, 1-11. doi.org/10.1002/bies.2017001066
ACUÑA OS, AVILÉS M, GUILLÉN-MARTÍNEZ A, LÓPEZ-ÚBEDA R, SORIANO-ÚBEDA C, COY P, IZQUIERDO-RICO MJ. Differential oviductal gene expression in porcine estrous cycle. Reproduction, Fertility and Development. (2017) 29:2387-2399. doi.org/10.1071/RD164576
CÁNOVAS S, IVANOVA E, ROMAR R, GARCÍA-MARTÍNEZ S, SORIANO-ÚBEDA C, GARCÍA VÁZQUEZ FA, SAADEH H, ANDREWS S, KELSEY G, COY P. DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids. eLife. (2017) doi.org/10.7554/eLife.23670
LÓPEZ-ÚBEDA R, MUÑOZ M, VIEIRA L, HUNTER RHF, COY P, CANOVAS S. The oviductal transcriptome is influenced by a local ovarian effect in the sow. Journal of Ovarian research. (2016) doi.org/10.1186/s13048-016-0252-9
ALGARRA B, HAN L, SORIANO-ÚBEDA C, AVILÉS M, COY P, JOVINE L, JIMÉNEZ-MOVILLA M. The C-terminal region of OVGP1 remodels the zona pellucida 1 and modifies fertility parameters. Scientific Reports. (2016) 6:32556. doi.org/10.1038/srep32556
ROMAR R, FUNAHASHI H, COY P. In vitro fertilisation in pigs: new molecules and protocols to consider in the forthcoming years. Theriogenology. (2016) 85:125-34. doi.org/theriogenology.2015.07.017
LOPERA-VÁSQUEZ R, HAMDI M, MAILLO V, LLOREDA V, COY P, GUTIÉRREZ-ADÁN A, BERMEJO-ÁLVAREZ P, RIZOS D. Effect of bovine oviductal fluid on development and quality of bovine embryos in vitro. Reproduction Fertility and Development. (2015) 29:621-629. doi.org/10.1071/RD15238
COY P, YANAGIMACHI R. The common and species-specific roles of oviductal proteins in mammalian fertilization and embryo development. Bioscience. (2015) 65(10): 973-984. doi.org/10.1093/biosci/biv119
LÓPEZ-ÚBEDA R, GARCÍA-VÁZQUEZ FA, ROMAR R, GADEA J, MUÑOZ M, HUNTER RHF, COY P. Oviductal transcriptome is modified after insemination during spontaneous ovulation in the sow. PLoS One. (2015) doi.org/journal.pone.0130128
ROMERO-AGUIRREGOMEZCORTA J, MATÁS C, COY P. α-L-fucosidase enhances capacitation-associated events in porcine spermatozoa. Veterinary Journal. (2015) 203:109-114. doi.org/j.tvjl.2014.11.006
ROMERO-AGUIRREGOMEZCORTA J, SANTA AP, GARCÍA-VÁZQUEZ FA, COY P, MATÁS C. Nitric oxide synthase (NOS) inhibition during porcine in vitro maturation modifies oocyte protein S-nitrosylation and in vitro fertilization. PLoS One. (2014) doi.org/journal.pone.0115044
BALLESTER L, ROMERO-AGUIRREGOMEZCORTA J, SORIANO-ÚBEDA C, MATÁS C, ROMAR R, COY P. Timing of oviductal fluid collection, steroid concentrations and sperm preservation method affect in vitro fertilization efficiency . Fertility and Sterility. (2014) 102:1762-1768. doi.org/j.fertnstert.2014.08.009
SAAVEDRA MD, MONDÉJAR I, COY P, BETANCOURT M, GONZÁLEZ-MÁRQUEZ H, JIMÉNEZ-MOVILLA M, AVILÉS M, ROMAR R. Calreticulin from suboolemmal vesicles affects membrane regulation of polyspermy. Reproduction. (2014) 147 (3): 369–378. doi.org/10.1530/REP-13-0454
MONDÉJAR I, MARTÍNEZ I,AVILÉS M, COY P. Identification of potential oviductal factors responsible of the zona pellucida hardening and monospermy during fertilization in mammals. Biology of Reproduction. (2013) 89 (3): 67, 1-8. doi.org/biolreprod.113.111385
MONDÉJAR I, AVILÉS M, COY P. The human is an exception to the evolutionarily-conserved phenomenon of pre-fertilization zona pellucida resistance to proteolysis induced by oviductal fluid. Human Reproduction. (2013) 28:718-728. doi.org/10.1093/humrep/des423
GRULLÓN LA, GADEA J, MONDÉJAR I, MATÁS C, ROMAR R, COY P. How is plasminogen/plasmin system contributing to regulate sperm entry into the oocyte? Reproductive Sciences. (2013) 20:1075-1082. doi.org/10.1177/1933719112473657
COY P, GARCÍA-VÁZQUEZ FA, VISCONTI P, AVILÉS M. Roles of the oviduct in mammalian fertilization. Reproduction. (2012) 144 649–660. doi.org/10.1530/REP-12-0279
ROMAR R, COY P, RATH D. Maturation conditions and boar affect timing of cortical reaction in porcine oocytes. Theriogenology. (2012) 178:1126-1139. doi.org/j.theriogenology.2012.05.009
CEBRIÁN-SERRANO A, SALVADOR I, GARCIA-ROSELLÓ E, PERICUESTA E, PÉREZ-CEREZALES S, GUTIERREZ-ADÁN A, COY P, SILVESTRE MA. Influence of oviductal fluid on in vitro fertilisation, development and gene expression of in vitro produced bovine blastocysts. Reproduction in Domestic Animals. (2012) 48(2): 331-338. doi.org/j.1439-0531.2012.02157.x
COY P, JIMÉNEZ-MOVILLA M, GARCÍA-VÁZQUEZ FA, MONDÉJAR I, GRULLÓN L, ROMAR R. Oocytes use plasminogen-plasmin system to remove supernumerary spermatozoa. Human Reproduction. (2012) 27(7):1985-1993. doi.org/10.1093/humrep/des146
MONDÉJAR I, ACUÑA S, IZQUIERDO RICO MJ, COY P, AVILÉS M. The oviduct: functional genomic and proteomic approach. Reproduction in Domestic Animals. (2012) 47(3): 22-29. doi.org/j.1439-0531.2012.02027.x
MONDÉJAR I, GRULLÓN LA, GARCÍA-VÁZQUEZ FA, ROMAR R, COY P. Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen- activators during interplay between gametes. Fertility and Sterility. (2012) 97(2):453-461. doi.org/j.fertnstert.2011.11.032
HUNTER RHF, COY P, GADEA J, RATH D. Consideration of viscosity in the preliminaries to mammalian fertilisation. Journal Assisted Reproduction and Genetics. (2011) 28(3):191-7. doi.org/s10815-010-9531-3
AVILES M, GUTIERREZ-ADAN A, COY P. Oviductal secretions: will they be key factors for the future ARTs? Molecular Human Reproduction. (2010) 16(12): 896-906. doi.org/10.1093/molehr/gaq056
COY P, AVILES M. What controls polyspermy in mammals, the oviduct or the oocyte? Biological Reviews Camb Philos Soc. (2010) 85(3):593-605. doi.org/j.1469-185x.2009.00117.x
COY P, LLOYD R, ROMAR R, SATAKE N, MATAS C, GADEA J, HOLT WV. Effects of porcine pre-ovulatory oviductal fluid on boar sperm function. Theriogenology. (2010) 74(4):632-642. doi.org/j.theriogenology.2010.03.005
MATAS C, SANSEGUNDO M, RUIZ S, GARCIA-VAZQUEZ FA, GADEA J, ROMAR R, COY P. Sperm treatment affects capacitation parameters and penetration ability of ejaculated and epididymal boar spermatozoa. Theriogenology. (2010) 74:1327–1340. doi.org/j.theriogenology.2010.06.002
COY P, GADEA J, RATH D, HUNTER RHF. Differing sperm ability to penetrate the oocyte in vivo and in vitro as revealed using colloidal preparations. Theriogenology. (2009) 72:1171–1179. doi.org/j.theriogenology.2009.07.011
CÁNOVAS S, ROMAR R, GRULLON LA, AVILES M, COY P. Pre-fertilization zona pellucida hardening by different cross-linkers affects in vitro fertilization and embryo production in pigs and cattle. Reproduction. (2009) 137:803-812. doi.org/10.1530/REP-08-0340
LLOYD R, ROMAR R, MATAS C, GUTIÉRREZ-ADÁN A, HOLT WV, COY P. Effects of oviductal fluid on the generation, quality and gene expression of porcine blastocyst produced in vitro. Reproduction. (2009) 137:679-687. doi.org/10.1530/REP-08-0405
CAMPOS I, GOMEZ E, FERNANDEZ-VALENCIA AL, LANDERAS J, GONZALEZ R, COY P, GADEA J. Effects of male and recipient age on the reproductive outcome of an oocyte donation program. Journal Assisted Reproduction and Genetics. (2008) 25:445-452. doi.org/s10815-008-9255-95
CANOVAS S, COY P. Aspectos moleculares de la fecundación: unión y fusión de gametos. Revista de Investigación clínica. (2008) 60:403-413. Link to Article
CARRASCO LC, COY P, AVILES M, GADEA J, ROMAR R. Glycosidase determination in bovine oviduct fluid at follicular and luteal phases of the estrous cycle. Reproduction, Fertility and Development . (2008) 20 1-10. doi.org/10.1071/rd08113
CARRASCO LC, ROMAR R, AVILES M, GADEA J, COY P. Determination of glycosidase activity in porcine oviduct fluid at the different phases of the estrous cycle. Reproduction. (2008) 136: 833–842. doi.org/10.1530/REP-08-0221
COY P, CÁNOVAS S, ROMAR R, SAAVEDRA MD, GRULLÓN L, MONDÉJAR I, MATÁS C, AVILÉS M. Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during mammalian fertilization. Proc Nat Acad Sci USA (PNAS). (2008) 105:15809–15814. doi.org/10.1073/pnas.0804422105
RATH D, SCHUBERTH HJ, COY P, TAYLOR U. Sperm interactions from insemination to fertilisation. Reproduction in Domestic Animals. (2008) 43 (Suppl.5) 2-11. doi.org/j.1439-0531.2008.01250.x
COY P, GRULLÓN LA, CANOVAS S, Romar R, AVILES M, MATAS C. Hardening of the zona pellucida of unfertilized eggs can reduce polyspermic fertilization in the pig and cow. Reproduction. (2008) 135:19-27. doi.org/10.1530/rep-07-0280
GARCÍA-ROSELLÓ E, GARCÍA-MENGUAL E, COY P, ALFONSO J, SILVESTRE MA. Intracytoplasmic sperm injection in livestock species: an update. Reproduction in Domestic Animals. (2009) 44:143-151. doi.org/j.1439-0531.2007.01018.x
CANOVAS S, COY P, GOMEZ E. First steps for the development of a functional assay for human sperm using pig oocytes. Journal of Andrology. (2007) 28(2):273-81. doi.org/jandrol.106.001081
VELÁSQUEZ JG, CÁNOVAS S, BARAJAS P, MARCOS J, JIMÉNEZ-MOVILLA M, GUTIÉRREZ GALLEGO R, BALLESTA J, AVILÉS M, COY P. Role of sialic acid in bovine sperm-zona pellucida binding. Molecular Reproduction and Development. (2007) 74(5):617-28. doi.org/10.1002/mrd.20619
GARCÍA ROSELLÓ E, COY P, GARCÍA VÁZQUEZ FA, RUIZ S, MATÁS C. Analysis of different factors influencing the Intracytoplasmic sperm Injection (ICSI) yield in pigs. Theriogenology. (2006) 66(8):1857-1865. doi.org/j.theriogenology.2006.04.037
GARCÍA-ROSELLÓ E, MATÁS C, CANOVAS S, MOREIRA PN, GADEA J, COY P. Influence of sperm pretreatment on the efficiency of Intracytoplasmic Sperm Injection (ICSI) in pigs. Journal of Andrology. (2006) 27 268-275. doi.org/10.2164/jandrol.05110
COY P, ROMAR R, RUIZ S, CANOVAS S, GADEA J, GARCIA-VAZQUEZ F, MATAS C. Birth of piglets after transferring of in vitro produced embryos pre-matured with R-roscovitine. Reproduction. (2005) 29(6): 747-755. doi.org/10.1530/rep.1.00691
COY P, ROMAR R, PAYTON RR, MCCANN L, SAXTON AM, EDWARDS JL. Maintenance of meiotic arrest in bovine oocytes using the S-enantiomer of roscovitine: Effects on maturation, fertilization and subsequent embryo development in vitro. Reproduction. (2005) 129(1): 19-26. doi.org/10.1530/rep.1.002991
ROMAR R, COY P, GADEA J, RATH D. Effect of oviductal and cumulus cells on zona pellucida and cortical granules of porcine oocytes fertilized in vitro with epididymal spermatozoa. Animal Reproduction Science. (2005) 85(3-4): 287-300. doi.org/j.anireprosci.2004.04.0401
MATÁS C, GARCÍA-VÁZQUEZ FA, SANSEGUNDO M, GADEA J, COY P, RUIZ S. Estudio de la capacitación espermática in vitro en espermatozoides eyaculados y epididimarios. ITEA. (2007) 28:30-32.
GADEA J, SELLÉS E, MARCO MA, COY P, MATÁS C, ROMAR R, RUIZ S. Decrease in glutatione content in boar sperm alter cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology. (2004) 62:690-701. doi.org/j.theriogenology.2003.11.013
PAYTON BP, ROMAR R, COY P, SAXTON AM, LAWRENCE JL, EDWARDS JL. Susceptibility of bovine germinal vesicle-stage oocytes from antral follicles to direct effects of heat stress in vitro. Biology of Reproduction. (2004) 71: 1303–1308. doi.org/biolreprod.104.029892
MATÁS C, COY P, ROMAR R, GADEA J, RUIZ S. Effect of sperm preparation method on in vitro fertilisation in the pig. Reproduction. (2003) 125: 133-141. doi.org/10.1530/rep.0.1250133
ROMAR R, COY P, RUIZ S, GADEA J, RATH D. Effect of oviductal and cumulus cells on in vitro fertilization and embryo development of porcine oocytes fertilized with epidymal spermatozoa. Theriogenology. (2003) 125: 133-141. doi.org/S0093-691X(02)01138-X
COY P, GADEA J, ROMAR R, MATÁS C, GARCÍA E. Effect of in vitro fertilization medium on the acrosome reaction, cortical reaction, zona pellucida hardening and in vitro development in the pig. Reproduction. (2002) 124: 279-288. doi.org/10.1530/rep.0.1240279
COY P, ROMAR R. In vitro production of pig embryos. A point of view. Reproduction, Fertility and Development. (2002) 14:275-286. doi.org/10.1071/rd01102
CAMPOS I, COY P, ROMAR R, RUIZ S, GADEA J. Effect of maturational stage, coincubation and cumulus cells on in vitro penetrability of porcine oocytes. Theriogenology. (2001) 55: 1489-1500. doi.org/S0093-691X(01)00496-4
ROMAR R, COY P, CAMPOS I, GADEA J, MATÁS C, RUIZ S. Effect of co-culture with porcine oviductal epithelial cells (POEC) on pig in vitro fertilization. Animal Reproduction Science. (2001) 68: 85-98. doi.org/s0378-4320(01)00133-6
COY P, RUIZ S, ROMAR R, CAMPOS I, GADEA J. Maturation, fertilization and full development of porcine oocytes matured under different systems. Theriogenology. (1999) 51: 799-812. doi.org/S0093-691X(99)00028-X
COY P, MARTÍNEZ E, RUIZ S, VÁZQUEZ JM, ROCA J, GADEA J. Environment and medium volume influence in vitro fertilisation of pig oocytes. Zygote. (1993) 1: 209-213. doi.org/S0967199400001489
COY P, MARTÍNEZ E, RUIZ S, VÁZQUEZ JM, ROCA J, MATÁS C, PELLICER MT. In vitro fertilization of pig oocytes after different coincubation intervals. Theriogenology. (1993) 39: 1201-1208. doi.org/0093-691X(93)90018-Z
COY P, MARTÍNEZ E, RUIZ S, VÁZQUEZ JM, ROCA J, MATÁS C. Sperm concentration influences fertilization and male pronuclear formation in vitro in pigs. Theriogenology. (1993) 40: 539-546. doi.org//0093-691X(93)90407-V
MARTÍNEZ E, VÁZQUEZ JM, MATÁS C, ROCA J, COY P, GADEA J. Evaluation of boar spermatozoa penetrating capacity using pig oocytes at the germinal vesicle stage. Theriogenology. (1993) 40: 547-557. doi.org/0093-691X(93)90408-W
VÁZQUEZ JM, MARTÍNEZ E, ROCA J, COY P, PASTOR LM. Evaluation of boar spermatozoa penetrating capacity using pig oocytes at the germinal vesicle stage. Molecular Reproduction and Development. (1993) 36:84-88. doi.org/10.1002/mrd.1080360112
ROCA J, MARTÍNEZ E, VÁZQUEZ JM, COY P. Characteristics and seasonal variations in the semen of Murciano-granadina goats in the Mediterranean area. Animal Reproduction Science.(1992) 29:255-262. doi.org/0378-4320(92)90038-F
VÁZQUEZ JM, MARTÍNEZ E, ROCA J, COY P, RUIZ S. Use of triple stain technique for simultaneous assessment of vitality and acrosomal status in boar spermatozoa. Theriogenology. (1992) 38:843-852. doi.org/0093-691X(92)90160-S
ROCA J, MARTÍNEZ E, VÁZQUEZ JM, RUIZ S, COY P. Influence of season and photoperiod on testicular size and libido in male goats from the Mediterranean area. Animal Production. (1991) 93:10-14. doi.org/10.1017/S0003356100012836
Research Projects and Grants
Research Projects and Grants (from 2007)
Substituting Mouse Embryo Assay by Bovine Embryo Assay (or how to save one million mice per year in Europe). BEA-for-MEA.
Funding body: Proyectos en Líneas Estratégicas del Ministerio de Ciencia e Innovación - Referencia: PLEC2022-009246.
Participating Institutions: Universidad de Murcia, Centro de Investigación Príncipe Felipe, Embryocloud SL y Sinae SL.
Duration: 01-11-2022 - 31-10-2025
PI: Pilar Coy Fuster
Amount granted: 722.890,64 €
Validación y escalado de las técnicas de edición genética con aplicaciones en el ámbito biomédico y ganadero.
Funding body: Ayudas a la realización de proyectos para el desarrollo de investigación científica y técnica por grupos competitivos 2022. Fundación Seneca. Ref. 22065/PI/22.
Duration: 01/01/23 - 31/12/2025
PI: Joaquin Gadea
Amount granted: 103.400,00 €
Phenotypic and genetic characterization of livestock animals derived from assisted reproductive technologies (PhenotypART).
Funding body: Ministerio de Ciencia e Innovación. PID2020-113366RB-I00
Duration: 2021 - 2024
PI: Raquel Romar y Pilar Coy
Amount granted: 223.850 € (resolución provisional, pendiente alegaciones)
From assisted reproductive technologies onwards: their impact on preimplantation epigenetic reprogramming and their use in the generation of CRISPR-Cas9 transgenic animals.
Funding body: Agencia de Ciencia y Tecnología de la Región de Murcia. Fundación Séneca Ayudas a los Grupos y Unidades de Excelencia Científica de la Región de Murcia. 20040/GERM/16
Duration: 2016 - 2019
PI: Pilar Coy and Joaquin Gadea
Amount granted: 200.000 €
Obtaining healthy offspring by assisted reproductive technologies mimicking physiological conditions.
Funding body: Ministerio de Economía y Competitividad. AGL2015-66341-R
Duration: 2016 - 2019
PI: Pilar Coy and Joaquin Gadea
Amount granted: 272.250 €
Marie Sklodowska-Curie Innovative Training Network in Biology and Technology of Reproductive Health.
Funding body: European commission. Research Executive Agency (REA). Rep-Biotech 675526
Duration: 2015 - 2019
PI: Pilar Coy
Amount granted: 3.811.469,04 €
Physic-chemical, molecular and cellular characterization of the conditions that determine the fertilization success in mammals.
Subproject 1: Description of the new physiological pathways participating in mammalian fertilization.
Funding body: Ministerio de Economía y Competitividad. AGL2012-40180-C03-01
Duration: 2013 - 2015
PI: Pilar Coy
Amount granted: 216.450 €
Genomic study of the porcine oviduct in relation with interactions produced during fertilization.
Funding body: Fundación Séneca. Centro de Coordinación de la Investigación, Comunidad Autónoma Región de Murcia. REF: 11996/PI/09.
Duration: 2010 - 2015
PI: Pilar Coy
Amount granted: 45.944 €
Generation of new knowledge about the biological mechanism of fertilization and the application of the results to the improvement of IVF in mammals.
Funding body: Ministerio de Ciencia e Innovación. AGL2009-12512-C02-01
Duration: 2010 - 2012
PI: Pilar Coy
Amount granted: 227.480 €
Identification of molecules involved in the polyspermy blocking mechanism in bovine and porcine species. Application on the improvement of IVF output, design of a new culture medium and the production of transgenic pigs.
Funding body: Ministerio de Educación y Ciencia. AGL2006-03495
Duration: 2007 - 2009
PI: Pilar Coy
Amount granted: 218.526 €
Industry Sector and Patents
Contracts with the industry sector
| Title: | Puesta a punto en la Universidad de Murcia, de un santuario de cerdos producidos mediante técnicas de reproducción asistida para su estudio como modelos de enfermedades humanas. |
|---|---|
| Company: | CEFUSA |
| Duration: | 23/05/2019 - 23/05/2029 |
| PI: | Pilar Coy |
| Amount granted: | 55.000 € |
| Title: | New protocol for in vitro embryo culture and the creation of the first donated uterine fluids collection. |
|---|---|
| Company: | IVI Murcia |
| Duration: | 29/05/2017 - 29/05/2020 |
| PI: | Pilar Coy |
| Amount granted: | 70.000 € |
| CDTI project: | IDI-20170306 |
| Title: | Development of two tools for the diagnosis of the embryonic implantation capacity and the sperm fertilization capacity in the human and an associated treatment protocol. |
|---|---|
| Company: | IVI Murcia |
| Duration: | 01/01/2013 - 27/07/2025 |
| PI: | Pilar Coy |
| Amount granted: | 66.000 € |
| CDTI project: | IDI-20120531 |
Patents
| Title: | Processing and use of fluids from the reproductive tract to improve in vitro embryo production in mammals. |
|---|---|
| Application number: | P201400811 |
| Priority date: | 08/10/2014 |
| Inventors: | Raquel Romar and Pilar Coy |
| Applicant: | University of Murcia |
| Patent number: | ES 2532659B2 (PCT and USA extensions approved) |
| Publication date: | 30/03/2015 |
| Exploitation: | Licensed to Embryocloud SL (www.embryocloud.com). |
| Title: | Method for increasing monospermy during in vitro fertilization. |
|---|---|
| Application number: | P200602334 |
| Priority date: | 14/09/2006 |
| Inventors: | Pilar Coy and Manuel Avilés |
| Applicant: | University of Murcia |
| Patent number: | 2323993 |
| Publication date: | 31/05/2010 |
Networks
Networks membership
COST Action BM1308 SALAAM.
Sharing Advances on Large Animal Models.
Duration: 19/05/2014 - 18/05/2018
Chair: Eckhard Wolf
Web: COST_Action-BM1308
COST Action FA1201 EPICONCEPT.
Epigenetics and Periconception Environment.
Duration: 07/06/2012 - 06/06/2016
Chair: Ann Van Soom
Spain MC members: Alfonso Gutiérrez-Adán and Pilar Coy
Web: COST_Action-FA1201
COST Action FA0702 GEMINI.
Maternal Interaction with Gametes and Embryo.
Duration: 28/02/2008-27/02/2012
Chair: Alireza Fazeli
Web: COST_Action-FA0702
Stays
Research stays in foreign countries (last years)
| Center: | The Babraham Institute. Epigenetics Programme | University of Cambridge (UK) |
|---|---|
| Duration: | 22 weeks | 01/03/2015 - 31/07/2015 |
| Research topic: | Study of the whole genome methylation profile of bovine embryos produced in vitro by two different systems |
| Funded by: | Ayudas Movilidad Programa Jiménez de la Espada | Fundación Séneca. Comunidad Autonoma Región de Murcia |
| Center: | The Babraham Institute. Epigenetics Programme | University of Cambridge (UK) |
|---|---|
| Duration: | 26 weeks | 01/09/2014 – 28/02/2015 |
| Research topic: | Can the epigenetic alterations linked to the assisted reproductive technologies be avoided by introducing biofluids in the culture media? |
| Funded by: | Ayudas para movilidad de Profesores del Ministerio de Educación, Cultura y Deporte | Programa Salvador de Madariaga |
| Center: | Institute of Zoology. Zoological Society of London and University of Cambridge (UK) |
|---|---|
| Duration: | 38 weeks | 01/09/2006 – 30/05/2007 |
| Research topic: | Effect of oviductal proteins on the sperm and the oocyte |
| Funded by: | Ayudas para movilidad de Profesores del Ministerio de Educación, Cultura y Deporte |
Contact
Address
Dpto. Fisiología - Facultad de VeterinariaCampus Espinardo - Universidad de Murcia.
30100. Murcia. España.
Phone
+(34) 868 884789
pcoy@um.es
