About Us
The Tissue Culture Service (SCT) activity started in 1983 as a Core Facility of the Faculty of Medicine and nowadays is a member of the Área Científica y Técnica de Investigación (ACTI, Core Facility). From then until now, we have been collaborating with researchers from our University, as well as researchers from other centers and private companies, in the development of different techniques related to the cell and tissue culture.
The Tissue Culture Service is also a Core Facility of the Instituto Murciano de Investigación Biosanitaria (Murcian Institute of Biosanitary Research, IMIB).
The facilities have the confinement requirements for the development of activities that require a level 2 of biological containment (BSL2 laboratories) and also have the approval to work with OMG type 1 and 2.
The SCT, supports research by providing laboratoires, equipment, cell cultures, media, supplies, technical advice and expertise for their users. The SCT provides cultures for researchers, media and materials to support cell culture, and also allows use of laminar flow hoods, incubators, centrifuges, microscopes, refrigerators/freezers, and spacialized techniques related to cells in culture.
Services
The Cell Culture Service offers:
A. Specialized Technical Staff
- Expert consultation for researchers regarding cell culture needs, media, and experimental design
- Technical advice to the users.
B. BSL2 labs and equipment to carry out cell and tissue culture
C. Supervision of the ACTI Cell Culture Facility (SCT)
- Users access control (record of authorized users).
- Collaboration in the elaboration / updating of the rules for the use of the cell culture rooms. Supervision of the fulfillment of these rules.
- Assignment of equipment and shifts of use / maintenance of these equipment
- Preventive maintenance/ cleaning of specific equipment and so on.
- Training in aseptic and cell culture techniques
- Tested media, serum, and cell lines
- Mycoplasma screening and elimination
- Cell Authentication by STR analysis provided in conjunction with the ACTI's Molecular Biology Service (More Info)
- Maintenance and cryopreservation of a large cell line repository
- Maintenance of samples in liquid nitrogen
- Media and reagents preparation for researchers who are using cells provided by the facility, to use on site.
- Preparation of cells for experiments (chek service availability)
- Others (chek service availability)
C. Technologies related to cell culture
- Flow Cytometry (FCM)
- Sorting by FCM and magnetic beads
- Spectroscopy, Fluorescence and Luminiscence technologies
- Multiplex Assays
- Real-Time, live cell kinetic and phenotypic measures
- Luminometry for Circadian Biology
- Dry Ice Supply
- Others (chek service availability)
Request and Rates
- Request: by means of GSAI software
- Rates
Links
Staff / Contact
Contact: acti.sct@um.es