Antioxidants: Bioanalysis

Recent papers published in scientific journals on  enzymatic bioanalysis of Antioxidants.

Antioxidants: Bioanalytical Assays with Tyrosinase

• Garcia-Jimenez, A., Teruel-Puche, J. A., Berna, J., Rodriguez-Lopez, J. N., Tudela, J., Garcia-Canovas, F.

Action of tyrosinase on alpha and beta-arbutin: A kinetic study.

PLOS ONE, 12(5), 1-19-, 2017. https://dx.doi.org/10.1371/journal.pone.0177330

• Ortiz-Ruiz, C.V., Berna, J., Tudela, J., Varon, R., Garcia-Canovas, F.

Action of ellagic acid on the melanin biosynthesis pathway.

J. Dermatol. Sci. 82(2): 115-122, 2016. https://dx.doi.org/10.1016/j.jdermsci.2016.02.004

• C.V. Ortiz-Ruiz; M.B.D. Santos; J. Berna; J. Fenoll; P.A. Garcia-Ruiz; J. Tudela, F. Garcia-Canovas.

Kinetic characterization of oxyresveratrol as a tyrosinase substrate.

IUBMB Life 67, 828-836, 2015.  https://dx.doi.org/10.1002/iub.1439  

• Muñoz-Muñoz, J.L.; Garcia-Molina, F.; Ros, E.; Tudela, J.; García-Cánovas, F., Rodriguez-López, J.N.

Prooxidant and antioxidant activities of rosmarinic acid.

Journal of Food Biochemistry  37, 396-408, 2013. https://dx.doi.org/10.1111/j.1745-4514.2011.00639.x

• Muñoz-Muñoz, J.L.; García-Molina, F.; Varón, R.; Tudela, J.; García-Cánovas, F., Rodríguez-López, J.N.

Quantification of the antioxidant capacity of different molecules and their kinetic antioxidant efficiencies.

Journal of Agricultural and Food Chemistry, 58, 2062-2070, 2010. https://dx.doi.org/10.1021/jf9042024

• García-Molina, F.; Muñoz, J.L.; García-Molina, M.; Molina-Alarcón, M.; García-Ruiz, P.A.; Tudela, J.; Rodríguez-López, J. N.

Determination and applications of the molar absorptivity of phenolic adducts with captopril and mesna.

Journal of Agricultural and Food Chemistry, 57, 1143-1150, 2009. https://dx.doi.org/10.1021/jf803447g

• García-Molina, F.; Muñoz, J. L.; Varón, R.; Rodríguez-López, J.N.; García-Cánovas, F., Tudela, J.

     A review on spectrophotometric methods for measuring the monophenolase and diphenolase activities of tyrosinase.

     Journal of Agricultural and Food Chemistry, 55, 9739-9749, 2007. https://dx.doi.org/10.1021/jf0712301

• Muñoz, J.L.; Garcia-Molina, F.; Varon, R.; Rodriguez-Lopez, J.N.; Tudela, J.; Garcia-Cánovas, F.

Calculating molar absorptivities for quinones. Application to the measurement of tyrosinase activity.

Analytical Biochemistry, 351, 128-138, 2006. https://dx.doi.org/10.1016/j.ab.2006.01.011

• Peñalver, M.J.; Rodríguez-López, J.N.; García-Cánovas, F., Tudela, J.

Enzymatic method with polyphenol oxidase for the determination of cysteine and N-acetylcysteine.

J. Agric. Food Chem. 53, 6183-6189, 2005. https://dx.doi.org/10.1021/jf050197k

• Fenoll, L.G.; Rodríguez-López, J.N.; García-Molina, F.; García-Cánovas, F., Tudela, J.

Unification for the expresión of the monophenolase and diphenolase activities of tyrosinase.

International Union of Biochemistry and Molecular Biology: Life, 54, 137-141, 2002. https://dx.doi.org/10.1080/15216540214537

• Peñalver, M.J.; Rodríguez-López, J. N.; García-Molina, F., García-Cánovas, F, Tudela, J.

Method for the determination of molar absorptivities of thiol adducts formed from diphenolic substrates of polyphenol oxidase.

Analytical Biochemistry, 309, 180-185, 2002. https://dx.doi.org/10.1016/s0003-2697(02)00312-3

Antioxidants: Bioanalytical Methods with Peroxidase

• Cano, A.; Hernández-Ruiz, J.; García-Cánovas, F.; Acosta, M. and Arnao, M.B.

An end-point method for estimation of the total antioxidant activity in plant material.

Phytochemical Analysis 9, 196-202, 1998.

• Arnao, M. B.; Cano, A.; Hernández-Ruiz, J.; García-Cánovas, F. and Acosta, M..

Inhibition by L-ascorbic acid and other antioxidants of the 2,2´-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) oxidation catalyzed by peroxidase: a new approach for determining total antioxidant status of foods.

Anal. Biochem. 236, 255-261, 1996.

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