Proteinases

Some results of research activities of GENZ about proteinases and zymogens.

Congresses

• V. Ortiz, V. Tomas, R. Martinez-Gutierrez, F. Garcia-Canovas, J.Tudela.

Ferulic acid characterization as non competitive inhibitor of trypsin.

ANQUE-ICCE-BIOTEC-14 International Congress, Madrid, Spain, 1-4 July 2014.

• A.B. Cutillas, V. Ortiz, V. Tomas, R. Martinez-Gutierrez, M. Sanchez, J.Tudela.

Linoleic acid operates as mixed inhibitor of human leukocyte elastase.

Antioxidants 2013, Marrakech, Morocco, 26-28 June 2013.

• V. Ortiz, V. Tomas, R. Martinez-Gutierrez, F. Garcia-Canovas, J.Tudela.

p-Coumaric acid characterization as non competitive inhibitor of trypsin.

Antioxidants 2013, Marrakech, Morocco, 26-28 June 2013.

Papers

• Masiá-Perez, J.; Escribano, J.; Valero, E.; Arribas, E.; Garcia-Moreno, M.; Muñoz-Muñoz, J.L.; Gómez-Ladrón de Guevara, R.; Varon, R.

A general model for non-autocatalytic zymogen activation in the presence of two different and mutually exclusive inhibitors. II. Relative weight of activation and inhibition processes.

Journal of Mathematical Chemistry, 48, 635-652, 2010.

• Masiá-Perez, J.; Escribano, J.; Valero, E.; Arribas, E.; Garcia-Moreno, M.; Muñoz-Muñoz, J.L.; Gómez-Ladrón de Guevara, R.; Varon, R.

A general model for non-autocatalytic zymogen activation in the presence of two different and mutually exclusive inhibitors. I. Kinetic analysis.

Journal of Mathematical Chemistry, 48, 617-634, 2010.

• Muñoz-Lopez, A.; Sotos-Lamas, A.; Arribas, E.; Escribano, J.; Masia-Perer, J.; Muñoz-Muñoz, J.L.; Varón, R.

Kinetic analysis of a general model of activation of aspartic proteinase zymogens involving a reversible inhibitor. II. Contribution of the uni- and bimolecular activation routes.

The Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 157-163, 2007.

• Muñoz-López, A.; Sotos-Lomas, A.; Arribas, E.; Masia-Perez, J.; García-Molina, F.; García-Moreno, M. and Varón, R.

Kinetic análisis of a general model of activation of aspartic proteinase zymogens involving a reversible inhibitor. I. Kinetic Analysis.

The Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 147-155, 2007.

• Varón, R. García-Moreno, M. Valera- Ruiperez D.García-Molina, F, García-Cánovas F, Ladrón de Guevara, R.G., Masiá- Pérez, J. and Havsteen, B.H.

Kinetic analysis of a general model of activation of aspartic proteinase zymogens.

Journal of Theoretical Biology, 39, 238-252, 2007.

• Sidrach, L.; García-Cánovas, F.; Tudela, J. y Rodríguez-López, J.N.

Purification of cynarases from artichoke (Cynara scolymus L.): enzymatic properties of cynarase A.

Phytochemistry, 66,41-49, 2005.

• Espín, J. C. y Tudela, J.

Experimental approach for the kinetic study of unstable and site-directed irreversible inhibitors. Kinetic origin of the positive cooperativity arising from the inactivation of trypsin by p-amidinophenylmethanesulfonyl fluoride.

Biochemical Journal, 299, 29-35 1994.

• Espín, J. C. y Tudela, J.

Experimental approach for the kinetic study of unstable and site-directed irreversible inhibitors and its application to the inactivation of chymotrypsin by phenylmethylsulfonyl fluoride.

Journal of Enzyme Inhibition 7, 175-190, 1993.

• Varón, R., Manjabacas, M.C., García Moreno, M., Valero, E. y García Cánovas, F.

Kinetic behaviour of zymogen activation processes in presence of an inhibitor.

The Biochemical Journal 290, 463-470, 1993.

• Vázquez, A., Varón, R., Tudela, J. y García Cánovas, F.

Kinetic characterization of a model for zymogen activation: An experimental design and kinetic data analysis.

Journal Molecular Catalysis-B 79, 347-363, 1993.

• Manjabacas, M.C., Valero, E., García, M., García Cánovas, F., Rodríguez, J.N. y Varón, R.

Kinetic analysis of the control through inhibition of autocatalytic zymogen activation.

The Biochemical Journal 282, 583-587, 1992.

• Varón, R., Havsteen, B.H., García, M., Vázquez, A., Tudela, J. y García Cánovas, F.

Kinetics of the trypsinogen activation by enterokinase and/or trypsin: coupling of a reaction in which the trypsin acts on one of its substrates.

Journal of Molecular Catalysis-B 66, 409-419, 1991.

• Varón, R., Havsteen, B.H., Vázquez, A., García, M., Valero, E. y García Cánovas, F.

Kinetics of the trypsinogen activation by enterokinase and trypsin.

Journal of Theoretical Biology 145, 123-131, 1990.

• Varón, R., García Cánovas, F., García Carmona, F., Tudela, J., Román, A. y Vázquez, A.

Kinetics of a model for zymogens activation: The case of high activating enzyme concentrations.

Journal of Theoretical Biology 132, 51-59, 1988.

• Varón, R., García Cánovas, F., García Carmona, F., Tudela, J., García, M., Vázquez, A. y Valero, E.

Kinetic of a general model for enzyme activation through a limited proteolysis.

Mathematical Biosciences 87, 31-45, 1988.

• Varón, R., Román, A., García Cánovas, F. y García Carmona, F.

Transient phase kinetics of activation of human plasminogen.

Bulletin of Mathematical Biology 48 (2), 149-166,1986.

• Tudela, J., García Cánovas, F., García Carmona, F., Iborra, J.L. y Lozano, J.A.

Irreversible inhibition of trypsin by TLCK. A continous method for kinetic study of irreversible enzymatic inhibitors in the presence of substrate.

The Int. J. Biochem. Cell Biol. 18, 285-288, 1986.

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